The robots will be used for transporting clinical waste and dirty linen, delivering food and dispensing drugs.
Ahead of the opening in August, the robots are being tested at the 300 million pounds Forth Valley Royal Hospital in Larbert, Stirlingshire.
The robots will operate along a dedicated network of tunnels beneath the hospital. The human staff will use a hand held personal digital assistant (PDA) system to call the robots.
The robot will then move towards the lift, collect or deliver its item and return to the lift.
This technology will work with the help of sensors, which will direct the functions.
Elspeth Campbell, spokesperson of NHS Forth Valley, said the new system would help in preventing infections.
"Staff is very pleased. It is exciting to be the first in the UK to do this." the Sun quoted Campbell as saying.
"We know they work well in other hospitals elsewhere in the world. While it is new, we aren't nervous because we know it is a system that works well," Campbell added.